A Palette of Minimally Tagged Sucrose Analogues for Real?Time Raman Imaging of Intracellular Plant Metabolism

Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role energy metabolism; however, there are no analogues real-time imaging live cells. We have optimised a synthetic approach to prepare sucrose including very small (?50 Da or less) Raman tags fructose moiety. Spectroscopic analysis identified alkyne-tagged compound 6 as analogue recognised by endogenous transporters cells with higher intensity than other derivatives. Herein, we demonstrate application of first optical probe visualise uptake intracellular localisation plant using microscopy.